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From
gene to protein
revolutionary tools for molecular biology and protein expression
Based
upon an advanced information management system called orchestratorIMS,
NextGen Sciences has developed a new suite of products, which
integrate biology, software and hardware, enabling the user
to plan, coordinate, execute, monitor and track, successful
cloning and expression strategies.
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The
next generation of expression vector technology – a
novel platform, building on the advantages of existing systems,
which facilitates the production of recombinant baculoviruses
in a single step, cutting process times by up to 10 days.
Now available for both manual and automated applications.
more > |
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The
baculoworkstation™ is the
first precision automated platform for robust, reproducible,
high throughput baculovirus protein expression. The advanced
system has been designed to address the issues facing researchers
using insect cells for protein expression. The manual production
of recombinant baculoviruses is a labour-intensive, time consuming,
technically challenging process. The baculoworkstation automates
these processes, providing “out-of-the-box” baculovirus
protein expression and removing the need for protocol development
or robotics programming more>
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The
first information management system to link directly between
experiments planned in-silico and those performed manually
in the laboratory, enabling the user to plan, coordinate,
execute, monitor and track, successful cloning and expression
strategies
now available as a stand-alone software package. more>
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Fully
integrating orchestratorIMS with an optimised technology platform,
enabling the user to send programs of work directly to laboratory
robotics and run modular molecular biology processes automatically.
more>
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Beginning with DNA species
such as PCR products, ORF's or cDNA libraries, the expressionfactory
will plan, automate and track the entire process of sub-cloning,
expression and purification of hundreds of proteins in parallel.
Compatible with Gateway®, TOPO®, and traditional cloning
techniques, the system automatically performs molecular biology
processes in a seamless workflow, providing the highest level
of throughput and maximising efficiency and productivity.
more> |
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Processes
available to run virtually in orchestratorIMS, and perform
either manually as individual protocols on the expressionworkstation
platform, or fully integrated and run automatically as projects
by the expressionfactory include:
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Plasmid purification • PCR • Restriction digestion
(single and double digestion) •3’ recess fill-in
• Ligation • SAP & CIP treatment • Kinase
reaction • Klenow treatment • Taq fill-in and
5’A-Tailing • Gateway • TOPO • Traditional
cloning methods • E.coli Transformation • Cell
growth monitoring • Protein Expression • Cell
lysis • Separation of soluble and insoluble fractions
by centrifugation • Purification of soluble and resolubilised
proteins • Solubilisation of insoluble cell fractions
• Refolding trials on solubilised proteins • Protein
sample preparation for SDS-PAGE analysis • In vitro
translation • With the option to run all processes in
a "batch mode” |
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