Assay Sidebar
Order Testing ServicesContact us directly referencing product:
Email: info@nextgensciences.com US tel: 1-866-973-7914 
Ordering & Sample
Submission Form
Home – CSF Discovery
Technology Button
CSFassay-human(PD) A.1.0
CSFassay-human(PD) A.1.0
Human CSF Parkinson’s Disease Protein Assay 32-Plex
The CSFassay-human(PD) A.1.0 simultaneously measures the concentration of 32 proteins in human CSF samples.
This technically validated assay has immediate applications for biomarker discovery and early stage biomarker qualification in Parkinson’s disease. Independent of antibodies and developed using mass spectrometry based MRM technology, this assay provides a quantitative measure of all 32 proteins simultaneously and with high specificity and reproducibility.
 |
|
Requirements
|
Design Spec
|
Achieved Validation Spec
|
|
|---|---|---|---|
| Markers | >43 Protein Analytes | 80 Protein Analytes | |
| Sample | Human CSF | pass | |
| Quantification | Single Peptide, heavy internal standard | pass | |
| Sample Volume | ≤60 µL | 60 µL | |
| Precision | Repeatability %CV | ≤15 % Mean of All Analytes | 7.20% |
| ≤25 % Individual Analyte | ≤14.80% | ||
| Total %CV | ≤20 % Mean of All Analytes | 9.20% | |
| ≤30 % Individual Analyte | ≤22.80% | ||
| Range | Quantitative | Normal CSF concentration is between LLOQ and ULOQ | 77 proteins |
| Qualitative | Normal CSF concentration is greater than LOD | 3 proteins (CST3, ORM1, PTGDS) | |
| Linearity | >0.95 R2 Mean of all Analytes | >0.95 R2 Mean of all Analytes | |
| >0.90 R2 Individual Analyte | >0.90 R2 Individual Analyte | ||
| Accuracy | Quantitative | Spike Recovery = 100% ± 30% | Range: 79% – 125% |
| Qualitative | No requirement | CHI3L1 = 69% | |
| Sensitivity | Less than 8 ng/mL | pass | |
| Specificity | Quantitative | Dilution Linearity = 100% ± 30% | Range:79% – 130% |
| Qualitative | No requirement | pass | |
|
Gene Name
|
UniProtKB
|
Description
|
|---|---|---|
| AMBP | AMBP_HUMAN | Protein AMBP |
| APLP2 | APLP2_HUMAN | Amyloid-like protein 2 |
| APOA1 | APOA1_HUMAN | Apolipoprotein A-I |
| APOD | APOD_HUMAN | Apolipoprotein D |
| APOE | APOE_HUMAN | Apolipoprotein E |
| B2M | B2MG_HUMAN | Beta-2-microglobulin |
| C3 | CO3_HUMAN | Complement C3 |
| C9 | CO9_HUMAN | Complement component C9 |
| CFH | CFAH_HUMAN | Complement factor H |
| CHGA | CMGA_HUMAN | Chromogranin-A |
| CHGB | SCG1_HUMAN | Secretogranin-1 |
| CLEC3B | TETN_HUMAN | Tetranectin |
| CNTN1 | CNTN1_HUMAN | Contactin-1 |
| CNTN2 | CNTN2_HUMAN | Contactin-2 |
| CP | CERU_HUMAN | Ceruloplasmin |
| F2 | THRB_HUMAN | Prothrombin |
| GC | VTDB_HUMAN | Vitamin D-binding protein |
| KLK6 | KLK6_HUMAN | Kallikrein-6 |
| KNG1 | KNG1_HUMAN | Kininogen-1 |
| NCAM1 | NCAM1_HUMAN | Neural cell adhesion molecule 1 |
| NPTXR | NPTXR_HUMAN | Neuronal pentraxin receptor |
| PARK7 | PARK7_HUMAN | Protein DJ-1 |
| PCSK1N | PCSK1_HUMAN | ProSAAS |
| PLG | PLMN_HUMAN | Plasminogen |
| PON1 | PON1_HUMAN | Serum paraoxonase/arylesterase 1 |
| SCG2 | SCG2_HUMAN | Secretogranin-2 |
| SERPINA1 | A1AT_HUMAN | Alpha-1-antitrypsin |
| SERPINF1 | PEDF_HUMAN | Pigment epithelium-derived factor |
| SERPINI1 | NEUS_HUMAN | Neuroserpin |
| SPARCL1 | SPRL1_HUMAN | SPARC-like protein 1 |
| SPP1 | OSTP_HUMAN | Osteopontin |
| TF | TRFE_HUMAN | Serotransferrin |
|
Contact us directly referencing Product: CSFassay-human(PD) A.1.0
|
Email:
|
info@nextgensciences.com |
|
US Tel::
|
1-866-973-7914 |
|
EU Tel::
|
+44 (0)1462 685 788 |
or alternatively download, complete and send the following form to info@nextgensciences.com
|
Ordering & Sample Submission Form |
For advice on storage, and shipping of your human CSF samples please refer to the ordering and sample submission form above.
If your proteins of interest are not currently in our CSF panel we can develop a custom assay for you. Please contact us directly by email or telephone to arrange a discussion with our assay development team.