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CSFassay-human(MS) A.1.0
CSFassay-human(MS) A.1.0
Human CSF Multiple Sclerosis Disease Protein Assay 49-Plex
The CSFassay-human(MS) A.1.0 simultaneously measures the concentration of 49 proteins in human CSF samples.
This technically validated assay has immediate applications for biomarker discovery and early stage biomarker qualification in Multiple Sclerosis. Independent of antibodies and developed using mass spectrometry based MRM technology, this assay provides a quantitative measure of all 49 proteins simultaneously and with high specificity and reproducibility.
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Requirements
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Design Spec
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Achieved Validation Spec
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|---|---|---|---|
| Markers | >43 Protein Analytes | 80 Protein Analytes | |
| Sample | Human CSF | pass | |
| Quantification | Single Peptide, heavy internal standard | pass | |
| Sample Volume | ≤60 µL | 60 µL | |
| Precision | Repeatability %CV | ≤15 % Mean of All Analytes | 7.20% |
| ≤25 % Individual Analyte | ≤14.80% | ||
| Total %CV | ≤20 % Mean of All Analytes | 9.20% | |
| ≤30 % Individual Analyte | ≤22.80% | ||
| Range | Quantitative | Normal CSF concentration is between LLOQ and ULOQ | 77 proteins |
| Qualitative | Normal CSF concentration is greater than LOD | 3 proteins (CST3, ORM1, PTGDS) | |
| Linearity | >0.95 R2 Mean of all Analytes | >0.95 R2 Mean of all Analytes | |
| >0.90 R2 Individual Analyte | >0.90 R2 Individual Analyte | ||
| Accuracy | Quantitative | Spike Recovery = 100% ± 30% | Range: 79% – 125% |
| Qualitative | No requirement | CHI3L1 = 69% | |
| Sensitivity | Less than 8 ng/mL | pass | |
| Specificity | Quantitative | Dilution Linearity = 100% ± 30% | Range:79% – 130% |
| Qualitative | No requirement | pass | |
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Gene Name
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UniProtKB
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Description
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|---|---|---|
| A1BG | A1BG_HUMAN | Alpha-1B-glycoprotein |
| AGT | ANGT_HUMAN | Angiotensinogen |
| AHSG | FETUA_HUMAN | Alpha-2-HS-glycoprotein |
| APOA1 | APOA1_HUMAN | Apolipoprotein A-I |
| APOA4 | APOA4_HUMAN | Apolipoprotein A-IV |
| APOD | APOD_HUMAN | Apolipoprotein D |
| APOE | APOE_HUMAN | Apolipoprotein E |
| APOH | APOH_HUMAN | Beta-2-glycoprotein 1 |
| ATRN | ATRN_HUMAN | Attractin |
| Bri2-23 | ITM2B_HUMAN | Integral membrane protein 2B (23AA peptide released from ITM2B (BRI2)) |
| C2 | CO2_HUMAN | Complement C2 |
| C3 | CO3_HUMAN | Complement C3 |
| C8B | CO8B_HUMAN | Complement component C8 beta chain |
| C9 | CO9_HUMAN | Complement component C9 |
| CFH | CFAH_HUMAN | Complement factor H |
| CFHR2 | FHR2_HUMAN | Complement factor H-related protein 2 |
| CHGA | CMGA_HUMAN | Chromogranin-A |
| CHGB | SCG1_HUMAN | Secretogranin-1 |
| CHI3L1* | CH3L1_HUMAN | Chitinase-3-like protein 1 |
| CLEC3B | TETN_HUMAN | Tetranectin |
| CLU | CLUS_HUMAN | Clusterin |
| CNTN1 | CNTN1_HUMAN | Contactin-1 |
| CP | CERU_HUMAN | Ceruloplasmin |
| CST3* | CYTC_HUMAN | Cystatin-C |
| ENPP2 | ENPP2_HUMAN | Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 |
| F2 | THRB_HUMAN | Prothrombin |
| FN1 | FINC_HUMAN | Fibronectin |
| GC | VTDB_HUMAN | Vitamin D-binding protein |
| GSN | GELS_HUMAN | Gelsolin |
| HPX | HEMO_HUMAN | Hemopexin |
| IGFBP3 | IBP3_HUMAN | Insulin-like growth factor-binding protein 3 |
| IGHA1 | IGHA1_HUMAN | Ig alpha-1 chain C region |
| IGHM | IGHM_HUMAN | Ig mu chain C region |
| KLK6 | KLK6_HUMAN | Kallikrein-6 |
| KNG1 | KNG1_HUMAN | Kininogen-1 |
| NCAM1 | NCAM1_HUMAN | Neural cell adhesion molecule 1 |
| NPTXR | NPTXR_HUMAN | Neuronal pentraxin receptor |
| ORM1* | A1AG1_HUMAN | Alpha-1-acid glycoprotein 1 |
| PARK7 | PARK7_HUMAN | Protein DJ-1 |
| PLG | PLMN_HUMAN | Plasminogen |
| PON1 | PON1_HUMAN | Serum paraoxonase/arylesterase 1 |
| PTGDS* | PTGDS_HUMAN | Prostaglandin-H2 D-isomerase |
| RBP4 | RET4_HUMAN | Retinol-binding protein 4 |
| SCG2 | SCG2_HUMAN | Secretogranin-2 |
| SERPINF1 | PEDF_HUMAN | Pigment epithelium-derived factor |
| SPARCL1 | SPRL1_HUMAN | SPARC-like protein 1 |
| SPP1 | OSTP_HUMAN | Osteopontin |
| TF | TRFE_HUMAN | Serotransferrin |
| TTR | TTHY_HUMAN | Transthyretin |
* Qualitative protein
Order Human CSF Mulitple Sclerosis Assay Sample Testing
Contact us directly referencing Product: CSFassay-human(MS) A.1.0
|
Email:
|
info@nextgensciences.com |
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US Tel::
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1-866-973-7914 |
|
EU Tel::
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+44 (0)1462 685 788 |
or alternatively download, complete and send the following form to info@nextgensciences.com
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Ordering & Sample Submission Form |
For advice on storage, and shipping of your human CSF samples please refer to the ordering and sample submission form above.
If your proteins of interest are not currently in our CSF panel we can develop a custom assay for you. Please contact us directly by email or telephone to arrange a discussion with our assay development team.