ITEM
flashBAC
PART NUMBERS
P11578L - 4 reaction pack (20µl AT 20ng/µl)
P11579H - 24 reaction pack (120µl AT 20ng/µl)
P11580B - 100 reaction pack (500µl AT 20ng/µl)
P11581X - 96 well plate format (96 reactions) (480µl IN 5µl ALIQUOTS AT 20ng/µl)
flashBAC™ is a new technology for the production of recombinant baculoviruses. The system removes the need to separate recombinant virus from parental virus by plaque purification or any other means. flashBAC reduces the production of recombinant virus in insect cells to a one-step procedure.
The advantages of flashBAC include:
flashBAC lacks part of an essential gene (ORF 1629), preventing virus replication within insect cells. However, flashBAC also contains a bacterial artificial chromosome (BAC) at the polh locus, which allows the viral DNA to be maintained and propagated as a circular genome within bacterial cells.
Homologous recombination between flashBAC DNA and a transfer vector containing a gene of your choice restores the function of the essential gene and simultaneously removes the BAC sequence. The recombinant virus subsequently replicates to produce a genetically homogenous recombinant virus population without the need to perform a plaque assay.
After five days, virus can be added directly to insect cells to amplify a high titre working virus stock. This one-step procedure for making recombinant baculoviruses greatly facilitates the process of high throughput production of baculovirus expression vectors via automated systems.
flashBAC is further enhanced, by the deletion of the chitinase gene (chiA) thereby maximising the production of secreted and membrane-targeted proteins.
To find out more or to arrange a demonstration, call us now on:
UK & ROW: +44 (0) 1480 410 850
USA: 1-888-219-8471 (toll free)
Email: info@nextgensciences.com
