Gene to Protein

	Revolutionary tools for molecular biology and protein expression Based upon an advanced information management system called orchestratorIMS, NextGen Sciences has developed a new suite of products, which integrate biology, software and hardware, enabling the user to plan, coordinate, execute, monitor and track, successful cloning and expression strategies.

			The next generation of expression vector technology – a novel platform, building on the advantages of existing systems, which facilitates the production of recombinant baculoviruses in a single step, cutting process times by up to 10 days. Now available for both manual and automated applications. more >


			The baculoworkstation™ is the first precision automated platform for robust, reproducible, high throughput baculovirus protein expression. The advanced system has been designed to address the issues facing researchers using insect cells for protein expression. The manual production of recombinant baculoviruses is a labour-intensive, time consuming, technically challenging process. The baculoworkstation automates these processes, providing “out-of-the-box” baculovirus protein expression and removing the need for protocol development or robotics programming more>


			The first information management system to link directly between experiments planned in-silico and those performed manually in the laboratory, enabling the user to plan, coordinate, execute, monitor and track, successful cloning and expression strategies now available as a stand-alone software package. more>

			Fully integrating orchestratorIMS with an optimised technology platform, enabling the user to send programs of work directly to laboratory robotics and run modular molecular biology processes automatically. more>

			Beginning with DNA species such as PCR products, ORF's or cDNA libraries, the expressionfactory will plan, automate and track the entire process of sub-cloning, expression and purification of hundreds of proteins in parallel. Compatible with Gateway®, TOPO®, and traditional cloning techniques, the system automatically performs molecular biology processes in a seamless workflow, providing the highest level of throughput and maximising efficiency and productivity. more>

			Processes available to run virtually in orchestratorIMS, and perform either manually as individual protocols on the expressionworkstation platform, or fully integrated and run automatically as projects by the expressionfactory include: Plasmid purification PCR Restriction digestion (single and double digestion) recess fill-in Ligation SAP & CIP treatment Kinase reaction Klenow treatment Taq fill-in and 5’A-Tailing Gateway TOPO cloning Traditional cloning methods E.coli Transformation Cell monitoring Protein Expression Cell lysis Separation of soluble and insoluble fractions by centrifugation Purification of soluble and resolubilised proteins Solubilisation of insoluble cell fractions Refolding trials on solubilised proteins Protein sample preparation for SDS-PAGE analysis In vitro translation With the option to run all processes in a "batch mode”