Revolutionary tools for molecular biology and protein expression

Based upon an advanced information management system called orchestratorIMS, NextGen Sciences has developed a new suite of products, which integrate biology, software and hardware, enabling the user to plan, coordinate, execute, monitor and track, successful cloning and expression strategies.

The first information management system to link directly between experiments planned in-silico and those performed manually in the laboratory, enabling the user to plan, coordinate, execute, monitor and track, successful cloning and expression strategies
now available as a stand-alone software package.

Fully integrating orchestratorIMS with an optimised technology platform, enabling the user to send programs of work directly to laboratory robotics and run modular molecular biology processes automatically.

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  Processes available to run virtually in   orchestratorIMS, and perform either manually   as individual protocols on the   expressionworkstation platform, or fully   integrated and run automatically as projects   by the expressionfactory include:

• Plasmid purification
• PCR
• Restriction digestion
• (single and double digestion)
• recess fill-in
• Ligation
• SAP & CIP treatment
• Kinase reaction
• Klenow treatment
• Taq fill-in and 5’A-Tailing
• Gateway
• TOPO cloning
• Traditional cloning methods
• E.coli Transformation
• Cell monitoring
• Protein Expression
• Cell lysis
• Separation of soluble and insoluble fractions by centrifugation
• Purification of soluble and resolubilised proteins
• Solubilisation of insoluble cell fractions
• Refolding trials on solubilised proteins
• Protein sample preparation for SDS-PAGE analysis
• In vitro translation
• With the option to run all processes in a "batch mode”