discoveryexpress™
The service for discovery of biomarkers

Features of discoveryexpress™

Differential and quantitative protein profiling
High proteome and protein coverage
Easy transition to biomarker assay
Rapid turn-around time
Applicable to most biological sample types (human and animal biofluids, proximal fluids, tissues, cultured cells, formalin fixed parafilm embedded tissue, plants, etc)

View the discoveryexpress™ overview - PDF

Platforms used for discoveryexpress™

1. GeLC-MS

Features:	Process:
High number of poteins detected Provides information-rich proteome maps Rapid transition to protein assay Low sample requirements Fast turn-around time Low cost	Run each sample on a lane of a 1D SDS-PAGE gel Segment each lane (10, 24, 40 segments depending on sample complexity) In-gel digestion of proteins Mass spectrometry (LC-MS/MS) for identification and quantification of proteins Data analysis - list of potential biomarkers

2. Differential mass spectrometry (dMS, label-free mass spectrometry)

Features:	Process:
High throughput Amenable to large number of samples Focused on differentially expressed proteins Fractionation provides higher number of proteins with a lower throughput	Sample preparation, optional fractionation step, protein digestion Mass spectrometry (LC-MS/MS) Alignment of peptide chromatographic peaks Calculation of relative quantification Identification of differentially expressed proteins

3. iTRAQ

Features:	Process:
Comparison across 4 to 8 samples simultaneously	Sample preparation and protein digestion Label peptides with iTRAQ reagents Fractionation and mass spectrometry (LC-MS/MS Data analysis

4. SILAC (http://www.silac.org/)

Features:	Process:
Pair wise comparison of samples High number of proteins detected and quantified	Cells grown in medium containing deuterium, 13C, 15N to label proteins Sample preparation and fractionation Protein digestion and mass spectrometry (LC-MS/MS) Data analysis

5. Phosphoprotein analysis

Features:	Process:
Detection and quantification of phosphorylated proteins	Sample preparation Enrichment of phosphoproteins or phosphopeptides with antibodies and/or capture reagents Mass spectrometry (LC-MS/MS) Data analysis

Latest News

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Join NextGen Sciences at the Cancer Diagnostics: Next Generation Technologies on the 21st - 22nd August 2008, Washington, DC
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